Irf4a-gfp
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Irf4a-gfp
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Webccr9a Is a Direct Target of Irf4a (A) Co-expression of irf4a and ccr9a in the CHT of 2-dpf embryos revealed by double color FISH. Scale bar, 100 µm. (B) qPCR of irf4a and ccr9a in GFP + cells relative to negative cells sorted from coro1a :EGFP transgenic embryos at 2 dpf. p < 0.05; p < 0.01. (C) Schematic diagram of the ChIP and reporter assay. WebJun 5, 2024 · In fluorescence-activated cell sorting (FACS)-sorted hematopoietic progenitors (EMPs and HSCs), we found that kita was mildly enriched in HSCs but found a much higher enrichment of kitb in both EMPs and HSCs ( Figure 1 A), although HSC enrichment was not significant compared with the whole embryo ( Figure 1 A).
WebAug 19, 2015 · Ikaros-GFP high cells expressed high levels of gata3, cmyb, ccr9a, and irf4a. We therefore defined these cells as lymphoid-primed or lymphoid-committed HSCs ( Figures S4C-S4P), according to... WebApr 6, 2024 · Interferon regulatory factors (IRFs) constitute a family of transcription factors that synchronize interferon (IFN) antiviral response through translocating to nucleus and binding to the promoters of IFN and IFN-stimulated genes (ISGs).
An irf4a:GFP transgenic line was generated to mark and trace Irf4a-expressing cells (Figure S5D). In irf4a :GFP/ lyz :DsRed double transgenic embryos, the fate change of GFP + cells to DsRed + myeloid cells was not detectable in wild-type embryos but was evident in embryos upon irf4a knockdown ( Figure S5 E). WebThe p62/SQSTM1 (sequestosome 1) protein, welche acts as a cargo receptor for autophagic degradation of ubiquitinated targets, is up-regulated for assorted stressors. Induction of the p62 gene by oxidative stress the conveyed by NF-E2-related factor 2 (NRF2) and, at the same time, p62 protein contributes …
WebSep 28, 2015 · Generation of irf4a:GFP Transgenic Line. A 1.9-kb DNA fragment in the irf4a promoter region upstream of its start codon was amplified and cloned into the pGL3-GFP …
WebSep 28, 2015 · These data indicate that in addition to regulating ccr9a expression, Irf4a is essential in T lymphoid-primed progenitors for repressing Pu.1 expression to prevent an alternate fate. Our findings provide insight into the fate determination mechanism of T lymphoid-primed progenitors. Copyright © 2015 Elsevier Inc. All rights reserved. dxo iphoneWebApr 1, 2024 · Construction of irf4a transgenic zebrafish includes 6 steps. First off, amplify the CDS of irf4a and the sequence of "self-cleaving" 2A peptide. Then obtain the linearized … crystal notationWeb(D) Confocal images of irf4a-expressing cells in the CHT region of the irf4a:GFP transgenic embryos at 60 hpf (scale bar, 80 μm). The magnified view of irf4a-expressing cell is … crystal notebookWebIrf4a-Deficient Lymphoid Progenitors Turn to Myeloid Cells (A) Expression of coro1a in the siblings and irf4a mutants at 3 dpf. The black arrowhead indicates the thymus, and the red arrow indicates the coro1a -expressing cell in the CHT. crystal notary slidellWeb884 8 amjtransplant.comAm J Transplant.2024;19:884–893. 1 INTRODUCTION Organ transplantation is a life‐saving treatment for patients with end‐stage organ failure, but long‐term graft survival is limited by crystal notch filter design softwareWebPlasmid pTRIP-GFP-IRF3 from Dr. Nicolas Manel's lab contains the insert IRF3 and is published in Cell Rep. 2024 Feb 26;26(9):2377-2393.e13. doi: 10.1016/j.celrep.2024.01.105. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping ... crystal note cardsWebIrf4a is essential in T lymphoid-primed progenitors for repressing Pu.1 expression to prevent an alter- ... GFP erythroidcells(Fig-ure 1C). Double fluorescence in situ hybridization (FISH) further revealed that irf4a was co-expressed with cmyb (Figure 1D) but not with mpo (a neutrophil marker) or mfap4 (a macrophage marker) in the CHT region ... dxo iphone 12