WebSep 24, 2024 · CAMYEL detected concentration-dependent changes in cAMP activity at RXFP1-4 in recombinant cell lines, using a variety of ligands with potencies comparable to those seen in conventional cAMP … Webprocessing and analysis tools deliver a large assay window and improved sensitivity over methods relying on total fluorescence intensity measurements. Dual in-line dispense tips and rapid image capture rates support sequential addition of Gs- and Gi-coupled receptor agonists for detailed characterization of cAMP-dependent signaling pathways.
BRET Assays to Determine Altered Function of a D2DR Variant …
WebSep 26, 2024 · The assay is based on bioluminescence resonance energy transfer (BRET) that involves transfection of a biosensor in human embryonic kidney (HEK)-293 cells … WebSep 11, 2024 · CAMYEL assays. β 2 -adrenoceptor +/- 10 μM salbutamol was used as a positive control for constitutive cAMP signaling. Data represent n=3-4 assays performed in triplicate and final ligand concentrations were 10 μM. (c) CAMYEL assay performed as in (b) using CHO-K1 cells to control for cellular background; n=3 performed in triplicate. … shark washes up on long island
Identification and validation of modulators of exchange protein ...
WebHormone concentrations before and after semen-induced ovulation in the bactrian camel (Camelus bactrianus) Y. S. Xu, H. Y. Wang, G. Q. Zeng, G. T. Jiangand Y. H. Gao ... WebMeanwhile, PCR positive results (15%, 15%, and 20%) in male, Non-LF, and LF blood samples collected from camels were similar to those obtained using the LAMP assay when examining the same samples True positive Sensitivity = True positive+False negative × 100 Sensitivity LAMP assay on blood samples = 22/22 + 0 * 100 = 100% Sensitivity LAMP ... WebOct 1, 2024 · However, the CAMYEL assay was unable to detect the Gαi3‐mediated phase of RXFP1 cAMP activation in PTX‐treated THP‐1 cells or HEK293A cells with knockout of Gαs. shark watch band for iwatch